Story
- They begin in Gotham's North Burnley, where a gang war has erupted. New Villains are first enlisted to aid the Falcone Mob who are under siege by the Gotham Police Department. During the conflict they assist Catwoman in robbing a warehouse, despite interference from Huntress.
- At level 15, The Joker has his Villains lead a group of his men to attack the Gotham Police at a meeting in the East Regal Hotel, after driving the Police back they find The Joker locked in a fight with Batman who they distract long enough to allow The Joker's escape.
- Leading up to level 30, Tech Villains team up with The Joker to fight against Brainiac who has taken control of a satellite owned by the traitorous T.O. Morrow. The Villains manage to save The Joker and Harley Quinn from a modified form of their own toxin as well as an assault from Batman and Robin. With The Joker incapacitated they assault the satellite singlehandedly and save The Joker once again from a robotic duplicate of himself. The Tech story line culminates at level 30, when the player gets to meet with The Joker in person at the House of Legends for some heartfelt thanks and a hand shake.
Level 15: Punchline: Episode 2 - Instances
- Special Crimes Unit
- East End Regal Hotel
Level 30: Punchline: Episode 4 - Instances
- T.O. Morrow's Hideout
- Gotham S.T.A.R. Labs
- STAR Labs Satellite
- Undisclosed Location (Heroes)
- Arkham Asylum (Instance) (Villains)